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Registros recuperados: 360 | |
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Freitas,Cleverson D. T. de; Souza,Diego P. de; Araújo,Eliane S.; Cavalheiro,Mariana G.; Oliveira,Luciana S.; Ramos,Márcio V.. |
In this study, proteins extracted from laticifer cells of three plants were examined by electrophoresis, mass spectrometry (MALDI-TOF) and characterized in respect of proteolytic, chitinolytic and anti-oxidative activities by means of zymography and colorimetric assays. Acidic proteins with molecular masses between 12.5 and 74.5 kDa predominated in laticifers of P. rubra. This profile was not found in laticifers of C. grandiflora and E. tirucalli. The later was poor in respect of proteins. Strong anti-oxidative activity of superoxide dismutase (E.C. 1.15.1.1) was detected in P. rubra and C. grandiflora latices, and to a lesser extent ascorbate peroxidase (E.C. 1.11.1.1) and isoforms of peroxidase were seen. Catalase (E.C. 1.11.1.6) was detected only in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Catalase; Chitinase; Cysteine proteinase; Latex; Peroxidase. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202010000100002 |
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Pessoa,José Dalton Cruz; Calbo,Adonai Gimenes. |
According to the model frequently used as reference, plant cell growth occurs only when turgor surpasses a threshold. This model was proposed considering a cylindrical cell of constant wall thickness immerged in a water solution, with viscoelastic behaviour, hydraulic conductivity, variable extensibility, and unidirectional elongation. The author, Lockhart (1965), did not consider the effects of apoplasm hydrostatic potential, a subject treated later by Calbo and Pessoa (1994) who argued that this component of cell potential would interfere with cell growth rate. To evaluate this effect, where possible the same deductive procedures as those employed by Lockhart were used here, which resulted in a set of equivalent equations for cell growth, turgor and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoplasm; Cell wall; Growth; Lockhart; Model; Plant. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000100003 |
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Barata-Soares,Anderson D.; Gomez,Maria Luiza P. A.; Mesquita,Carlos Henrique de; Lajolo,Franco M.. |
Since the first isolation of ascorbic acid (AsA) in 1928, few papers have been published regarding the biosynthesis of AsA in plants, especially in fruits. It took as long as 1998, before Wheeler, Jones and Smirnoff, based on a study with Arabidopsis leaves, proposed what can be considered the main pathway of biosynthesis of AsA, in which L-galactose (L-GAL) is a key precursor. This paper reports the effectiveness of some precursors (cold or radiolabeled) in the biosynthesis of AsA in different plants: green sweet pepper, white-pulp guava, red-pulp guava, papaya and strawberry at two ripening stages (mature green and ripe for papaya and mature green and half red for strawberry) and broccoli. The 'Smirnoff-Wheeler' pathway was functioning and active in all... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ascorbate; Ascorbate biosynthesis pathway; Antioxidant; Precursor infiltration. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000300004 |
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Etienne,Hervé; Dechamp,E; Barry-Etienne,D; Bertrand,Bernóit. |
In coffee, bioreactors are the most promising way for scaling-up micropropagation processes, particularly somatic embryogenesis. The availability of an efficient somatic embryogenesis process would allow the rapid mass production of heterozygous materials such as selected Coffea canephora clones and F1 Arabica hybrid varieties. For the last fifteen years, bioreactors (mechanically or pneumatically agitated bioreactors, temporary immersion bioreactors) have mostly been used on coffee to optimize the mass regeneration of somatic embryos from embryogenic tissues. This review presents the main results, obtained with several bioreactor models, concerning the different steps of the micropropagation process : i) the multiplication of embryogenic tissues, ii) the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; Breeding; Liquid medium; Mass propagation; Somatic embryogenesis; Temporary immersion. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100005 |
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Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães. |
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; CDNA; EST; Transcriptome. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008 |
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Almeida,Julieta Andrea Silva de; Pereira,Maria de Fátima D. A.. |
O gênero Kohleria sp pertencente à família Gesneriaceae possui órgão subterrâneo. Este órgão é um caule envolto por folhas modificadas, imbricadas, as quais acumulam reservas e cada uma destas protege uma gema. Seções do rizoma brotam sem necessidade do órgão inteiro. Seções deste rizoma com seis folhas modificadas foram colocadas para brotar em placas de Petri e testadas quanto às temperaturas de 15, 20, 25, 30 e 35 ºC, na luz ou no escuro e ainda em relação aos teores de umidade do substrato, onde se adicionaram os volumes de 1, 12 e 20 mL de água destilada. Estes fatores influenciaram a brotação de seções do rizoma de Kohleria sp. As seções também se mostraram resistentes à falta de água, já que, nessa condição, apresentaram brotações e ainda as... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gema; Luz; Temperatura; Tempo de brotação; Teor de umidade. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100006 |
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Gonçalves,Jamile F.; Becker,Alexssandro G.; Cargnelutti,Denise; Tabaldi,Luciane A.; Pereira,Luciane B.; Battisti,Vanessa; Spanevello,Rosélia M.; Morsch,Vera M.; Nicoloso,Fernando T.; Schetinger,Maria R.C.. |
In this study, the effects of cadmium (Cd) on lipid peroxidation, electrolyte leakage, protein oxidation, ascorbate peroxidase (APX; E.C. 1.11.1.11), catalase (CAT; E.C. 1.11.1.6) and superoxide dismutase (SOD; E.C. 1.15.1.1) activities, and ascorbic acid, non-protein thiol groups and total soluble protein contents in cucumber seedlings (Cucumis sativus L.) were investigated. Seedlings were grown in vitro in an agar-solidified substrate containing four Cd levels as CdCl2 (0, 100, 400, and 1000 µmol L-1) for 10 d. The lowest Cd level decreased the malondialdehyde concentration. Electrolyte leakage increased only at 1000 µmol Cd L-1, whereas protein oxidation and total soluble protein content were enhanced at 400 and 1000 µmol Cd L-1. Activity of APX was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidant enzymes; Ascorbic acid; Cucumis sativus; Heavy metal; Lipid peroxidation; Protein oxidation. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300006 |
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Benavides,María P.; Gallego,Susana M.; Tomaro,María L.. |
Heavy metals are important environmental pollutants and their toxicity is a problem of increasing significance for ecological, evolutionary, nutritional, and environmental reasons. Plants posses homeostatic cellular mechanisms to regulate the concentration of metal ions inside the cell to minimize the potential damage that could result from the exposure to nonessential metal ions. This paper summarizes present knowledge in the field of higher plant responses to cadmium, an important environmental pollutant. Knowledge concerning metal toxicity, including mechanisms of cadmium homeostasis, uptake, transport and accumulation are evaluated. The role of the cell wall, the plasma membrane and the mycorrhizas, as the main barriers against cadmium entrance to the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cadmium; Heavy metals; Metal toxicity; Phytochelatins; Oxidative stress. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100003 |
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Vestena,Silvane; Cambraia,Jose; Ribeiro,Cleberson; Oliveira,Juraci A.; Oliva,Marco A.. |
The reactive oxygen species generation, lipid peroxidation and antioxidative enzyme response of water hyacinth and salvinia to Cd were evaluated. Cadmium was absorbed/accumulated mainly in the roots, but significant amounts also translocated to the leaves. No Cd effect on dry weight was detected, although toxicity symptoms were visible. Superoxide and H2O2 concentrations increased, in addition to lipid peroxidation in both species, especially in the leaves of salvinia. In general, antioxidative enzyme activities were reduced in both species following Cd treatment, especially in salvinia. Glutathione peroxidase (GPX, EC 1.11.1.9) activity decreased in water hyacinth but increased in salvinia. Glutathione S-transferase (GST, EC 2.5.1.18) activity increased... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aquatic plants; Eichhornia crassipes; Metal toxicity; Oxidative stress; Salvinia auriculata. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000200005 |
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Chaves,Ana Lúcia S.; Silva,J.A.; Pech,J.-C.; Latche,A.; Bouzayen,M.; Zegzouti,H.; Rombaldi,Cesar V.. |
O etileno, conhecido, sobretudo, como o fitormônio da maturação de frutos, está envolvido em vários processos fisiológicos, atuando na regulação da expressão de grande número de genes. O clone parcial de cDNA ER49 foi previamente isolado mediante a técnica do "Differential Display". A obtenção e a análise da seqüência completa confirmaram as homologias significativas com fatores de elongação da síntese de proteínas do tipo Ts. Tais proteínas são responsáveis pela reciclagem do complexo EF-TuGDP, que atua na ligação do aminoacil-RNAt ao ribossomo. A predição da seqüência protéica permitiu a detecção de um peptídio sinal de endereçamento para a mitocôndria e dos domínios TS-N e EF-Ts. A modelagem da estrutura terciária mostrou que ER49 poderia interagir com... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: EF-Ts; Etileno; Frutos climatéricos; Lycopersicon esculentum; Regulação pós-transcricional. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202002000100003 |
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Marchese,José Abramo; Broetto,Fernando; Ming,Lin Chau; Ducatti,Carlos; Rodella,Roberto Antonio; Ventrella,Marília Contin; Gomes,Greice Daiane Rodrigues; Franceschi,Lúcia de. |
Leaves of Artemisia annua L. are a plentiful source of artemisinin, a drug with proven effectiveness against malaria. The aim of this study was to classify the photosynthetic mechanism of A. annua through studies of the carbon isotope composition (delta 13C) and the leaf anatomy. A. annua presented a delta 13C value of - 31.76 ± 0.07, which characterizes the plants as a typical species of the C3 photosynthethic mechanism, considering that the average delta 13C values for C3 and C4 species are -28 and -14, respectively. The leaf anatomy studies were consistent with the delta 13C results, where, in spite of the existence of parenchymatic cells forming a sheath surrounding the vascular tissue, the cells do not contain chloroplasts or starch. This... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C3 and C4 plants; Isotope discrimination; Kranz anatomy; Photosynthesis. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202005000100016 |
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Gomes,Marcelo Pedrosa; Marques,Teresa Cristina Lara Lanza Sa e Melo; Martins,Giordane Augusto; Carneiro,Marília Mercia Lima Carvalho; Soares,Ângela Maria. |
Physiological and anatomical features of Cd-tolerance in Pfaffia glomerata were examined by exposing plantlets to nutrient solutions with increasing Cd concentrations (0, 15, 45, and 90 µmol Cd L-1), and possible Cd-tolerance markers were established. Cd contents were found to be higher in roots than in shoots. According to the bio-concentration factor data, this species is effectively a Cd-hyperaccumulator, as previously attested. Cd induced the appearance of xeromorphic characteristics in leaves (decreased water potential, increased numbers and decreased stomata size) and increased root endodermis thickness. The enzymatic antioxidant systems of roots and leaves were differently affected by Cd. The coordinated activities of antioxidant enzymes were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adaptation; Cd-oxidative damage; Phytoextraction; Tolerance. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202012000400008 |
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Registros recuperados: 360 | |
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